Fluorescence umpolung enables light-up sensing of N-acetyltransferases and nerve agents

نویسندگان

چکیده

Abstract Intramolecular charge transfer (ICT) is a fundamental mechanism that enables the development of numerous fluorophores and probes for bioimaging sensing. However, electron-withdrawing targets (EWTs)-induced fluorescence quenching long-standing unsolved issue in ICT fluorophores, significantly limits widespread applicability. Here we report simple generalizable structural-modification completely overturning intramolecular rotation driving energy, thus fully reversing fluorophores’ mode into light-up mode. Specifically, insertion an indazole unit scaffold can amplify donor-indazole-?-acceptor (fluorescence OFF), whereas efficiently suppressing their EWT-substituted system ON). This molecular strategy generalizable, yielding palette chromophores with umpolung spans visible near-infrared range. expands bio-analytical toolboxes allows exploiting sensing EWTs including N -acetyltransferases nerve agents.

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ژورنال

عنوان ژورنال: Nature Communications

سال: 2021

ISSN: ['2041-1723']

DOI: https://doi.org/10.1038/s41467-021-24187-5